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easyseptm human cd14 positive selection kit ii  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc easyseptm human cd14 positive selection kit ii
    Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from <t>CD14</t> + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .
    Easyseptm Human Cd14 Positive Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easyseptm+human+cd14+positive+selection+kit/easysep+human+t+cell+isolation+kit/pmc12268675-89-0-8
    Average 90 stars, based on 1 article reviews
    easyseptm human cd14 positive selection kit ii - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "HIV-1 infection of macrophages differentially primes NK-cell cytotoxicity and proinflammatory cytokine production"

    Article Title: HIV-1 infection of macrophages differentially primes NK-cell cytotoxicity and proinflammatory cytokine production

    Journal: iScience

    doi: 10.1016/j.isci.2025.112879

    Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from CD14 + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .
    Figure Legend Snippet: Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from CD14 + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .

    Techniques Used: Derivative Assay, In Vitro, Infection, Flow Cytometry, Staining, Expressing, Fluorescence

    Cell-to-cell contact and soluble priming factors on MDM are modulated by HIV-1 infection as well as NK cells (A) Experimental setup of MDM-NK cell co-culture. After 5 days of HIV-1 infection or 24 h of LPS/IFNγ-stimulation of MDM, autologous NK cells were added to the culture at a ratio of 1:1, according to the seeding density of CD14 + monocytes. After 24 h, NK-cell ligand expression on MDM was assessed. (B–D) MDM infection frequencies (CD4 − p24+ cells) after HIV-1 infection with 89.6 and THRO.c with and without 24 h NK-cell co-culture are depicted. Differences in infection frequencies between MDM with and without NK cell co-culture for each strain were analyzed using the Wilcoxon signed-rank test (89.6: n = 8; THRO.c: n = 9). Representative histogram of HLA-E (C) and HLA-ABC (D) surface expression on unstimulated MDM (left). Median fluorescence intensity (MdFI) of HLA-E and HLA-ABC on unstimulated, HIV-1-infected and bystander MDM is compared between MDM cultured without (NK-) or with NK cells (NK+) (right). Differences in MdFI values between infected and bystander MDM and between NK- and NK + MDM in each strain were analyzed using the Wilcoxon signed-rank test with FDR correction (Original Benjamini and Hochberg) (unstained (us): n = 19; unstim.: n = 21; 89.6 NK+: n = 14; 89.6 NK-: n = 9; THRO.c NK+: n = 11; THRO.c NK-: n = 10). (E–G) Scheme showing the cytokine analysis of supernatants from 24 h MDM-NK cell co-culture supernatants. Soluble IL-15 (F), IL-23 (G), and IL-18 (H, left) concentrations in MDM-NK cell co-culture supernatants from HIV-1 89.6, THRO.c and LPS/IFNγ conditions compared to unstimulated co-cultures. Differences in cytokine concentrations to unstimulated were analyzed using the Wilcoxon signed-rank test with FDR correction (Original Benjamini and Hochberg) (89.6: n = 4; THRO.c: n = 9; LPS/IFNγ: n = 10). Only significant p -values are shown. (H, right) Relation between IL-18 concentrations in 89.6- and THRO.c-infected MDM-NK co-cultures and the corresponding infection frequency. Spearman correlation coefficient was used to analyze the correlation between infection frequency and cytokine concentration (89.6: n = 4; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. Stainings were performed with antibody panels A and E (B, C right, D) and panel H (C left) . n.s., nonsignificant. See also .
    Figure Legend Snippet: Cell-to-cell contact and soluble priming factors on MDM are modulated by HIV-1 infection as well as NK cells (A) Experimental setup of MDM-NK cell co-culture. After 5 days of HIV-1 infection or 24 h of LPS/IFNγ-stimulation of MDM, autologous NK cells were added to the culture at a ratio of 1:1, according to the seeding density of CD14 + monocytes. After 24 h, NK-cell ligand expression on MDM was assessed. (B–D) MDM infection frequencies (CD4 − p24+ cells) after HIV-1 infection with 89.6 and THRO.c with and without 24 h NK-cell co-culture are depicted. Differences in infection frequencies between MDM with and without NK cell co-culture for each strain were analyzed using the Wilcoxon signed-rank test (89.6: n = 8; THRO.c: n = 9). Representative histogram of HLA-E (C) and HLA-ABC (D) surface expression on unstimulated MDM (left). Median fluorescence intensity (MdFI) of HLA-E and HLA-ABC on unstimulated, HIV-1-infected and bystander MDM is compared between MDM cultured without (NK-) or with NK cells (NK+) (right). Differences in MdFI values between infected and bystander MDM and between NK- and NK + MDM in each strain were analyzed using the Wilcoxon signed-rank test with FDR correction (Original Benjamini and Hochberg) (unstained (us): n = 19; unstim.: n = 21; 89.6 NK+: n = 14; 89.6 NK-: n = 9; THRO.c NK+: n = 11; THRO.c NK-: n = 10). (E–G) Scheme showing the cytokine analysis of supernatants from 24 h MDM-NK cell co-culture supernatants. Soluble IL-15 (F), IL-23 (G), and IL-18 (H, left) concentrations in MDM-NK cell co-culture supernatants from HIV-1 89.6, THRO.c and LPS/IFNγ conditions compared to unstimulated co-cultures. Differences in cytokine concentrations to unstimulated were analyzed using the Wilcoxon signed-rank test with FDR correction (Original Benjamini and Hochberg) (89.6: n = 4; THRO.c: n = 9; LPS/IFNγ: n = 10). Only significant p -values are shown. (H, right) Relation between IL-18 concentrations in 89.6- and THRO.c-infected MDM-NK co-cultures and the corresponding infection frequency. Spearman correlation coefficient was used to analyze the correlation between infection frequency and cytokine concentration (89.6: n = 4; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. Stainings were performed with antibody panels A and E (B, C right, D) and panel H (C left) . n.s., nonsignificant. See also .

    Techniques Used: Infection, Co-Culture Assay, Expressing, Fluorescence, Cell Culture, Concentration Assay

    Related Articles

    Isolation:

    Article Title: An immunocompetent human kidney on-a-chip model to study renal inflammation and immune-mediated injury.
    Article Snippet: PBMCswere thawed, taken up in Roswell Park Memorial Institute (RPMI) 1640 basal medium (11875093; Thermo Scientific) supplemented with 10% fetal bovine serum (FBS, 16140-071; Gibco), pelleted (300 g, 5 min), and washed with phosphate-buffered saline (PBS; 20012068; Sigma) containing 2% FBS. .. Monocytes were isolated fromPBMCs byCD14 positive selection using the EasySepTM Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according tomanufacturer’s protocol. .. In short, PBMCs were pelleted (300 g, 5 min) and resuspended in EasySepTM Buffer (20144; Stemcell Technologies) at a concentration of 1 × 108 cells ml−1 and incubated with 100 μl selection antibody cocktail per ml of sample for 10 min at RT.

    Article Title: Impact of donor extracellular vesicle release on recipient cell "cross-dressing" following clinical liver and kidney transplantation.
    Article Snippet: Conventional flow cytometry was performed using LSR Fortessa flow cytometer (BD) and analysed using FlowJo software v7.6 (Tree Star, CA). .. Monocytes from HLA-A2 negative healthy volunteers were isolated from PBMCs using the EasySepTM Human CD14 positive selection kit (Stemcell Technologies). .. Monocyte-derived DCs were generated by culturing freshly isolated monocytes for 5 days in EV-free complete media with 50 ng/mL of granulocyte-macrophage colony-stimulation factor (GM-CSF) and 800 U/mL IL-4 (R&D systems), at 37oC in 5% CO2.

    Article Title: Identification of Key Genes as Early Warning Signals of Acute Myocardial Infarction Based on Weighted Gene Correlation Network Analysis and Dynamic Network Biomarker Algorithm.
    Article Snippet: .. Leukocytes were isolated by centrifugation, and monocytes were isolated by using EasySepTM Human CD14 Positive Selection Kit (#18058, Stemcell, USA) following the manufacturer’s instructions. .. RNA was extracted from leukocytes by using TRIzol® Reagent (#15596018, Invitrogen, USA) and frozen at −80°C.

    Article Title: Acetaldehyde exposure underlies functional defects in monocytes induced by excessive alcohol consumption.
    Article Snippet: .. CD14+ monocytes were isolated by immunomagnetic positive selection using the EasySepTM Human CD14 Positive Selection Kit (STEMCELL technologies, Canada). ..

    Article Title: An immunocompetent human kidney on-a-chip model to study renal inflammation and immune-mediated injury
    Article Snippet: PBMCs were thawed, taken up in Roswell Park Memorial Institute (RPMI) 1640 basal medium (11875093; Thermo Scientific) supplemented with 10% fetal bovine serum (FBS, 16140-071; Gibco), pelleted (300g, 5 min), and washed with phosphate-buffered saline (PBS; 20012068; Sigma) containing 2% FBS. .. Monocytes were isolated from PBMCs by CD14 positive selection using the EasySepTM Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according to manufacturer’s protocol. .. In short, PBMCs were pelleted (300g, 5 min) and resuspended in EasySepTM Buffer (20144; Stemcell Technologies) at a concentration of 1 x 10 cells/mL and incubated with 100 μL selection antibody cocktail per mL of sample for 10 min at RT.

    Article Title: Acetaldehyde exposure underlies functional defects in monocytes induced by excessive alcohol consumption
    Article Snippet: The following antibodies were used for cell surface staining: PE-Cy7 Mouse Anti-Human CD14 (BD Pharmingen), APC-Cy7 Mouse Anti-Human CD16 (BD Pharmingen), FITC Mouse Anti-Human CD3 (BD Pharmingen), PE-Cy7 Mouse Anti-Human CD56 (BD Pharmingen), APC anti-human CD303 (BDCA-2) Antibody (BioLegend), and PerCP/Cyanine5.5 anti-human CD123 Antibody (BioLegend). .. CD14 + monocytes were isolated by immunomagnetic positive selection using the EasySepTM Human CD14 Positive Selection Kit (STEMCELL technologies, Canada). ..

    Selection:

    Article Title: An immunocompetent human kidney on-a-chip model to study renal inflammation and immune-mediated injury.
    Article Snippet: PBMCswere thawed, taken up in Roswell Park Memorial Institute (RPMI) 1640 basal medium (11875093; Thermo Scientific) supplemented with 10% fetal bovine serum (FBS, 16140-071; Gibco), pelleted (300 g, 5 min), and washed with phosphate-buffered saline (PBS; 20012068; Sigma) containing 2% FBS. .. Monocytes were isolated fromPBMCs byCD14 positive selection using the EasySepTM Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according tomanufacturer’s protocol. .. In short, PBMCs were pelleted (300 g, 5 min) and resuspended in EasySepTM Buffer (20144; Stemcell Technologies) at a concentration of 1 × 108 cells ml−1 and incubated with 100 μl selection antibody cocktail per ml of sample for 10 min at RT.

    Article Title: Impact of donor extracellular vesicle release on recipient cell "cross-dressing" following clinical liver and kidney transplantation.
    Article Snippet: Conventional flow cytometry was performed using LSR Fortessa flow cytometer (BD) and analysed using FlowJo software v7.6 (Tree Star, CA). .. Monocytes from HLA-A2 negative healthy volunteers were isolated from PBMCs using the EasySepTM Human CD14 positive selection kit (Stemcell Technologies). .. Monocyte-derived DCs were generated by culturing freshly isolated monocytes for 5 days in EV-free complete media with 50 ng/mL of granulocyte-macrophage colony-stimulation factor (GM-CSF) and 800 U/mL IL-4 (R&D systems), at 37oC in 5% CO2.

    Article Title: Identification of Key Genes as Early Warning Signals of Acute Myocardial Infarction Based on Weighted Gene Correlation Network Analysis and Dynamic Network Biomarker Algorithm.
    Article Snippet: .. Leukocytes were isolated by centrifugation, and monocytes were isolated by using EasySepTM Human CD14 Positive Selection Kit (#18058, Stemcell, USA) following the manufacturer’s instructions. .. RNA was extracted from leukocytes by using TRIzol® Reagent (#15596018, Invitrogen, USA) and frozen at −80°C.

    Article Title: Acetaldehyde exposure underlies functional defects in monocytes induced by excessive alcohol consumption.
    Article Snippet: .. CD14+ monocytes were isolated by immunomagnetic positive selection using the EasySepTM Human CD14 Positive Selection Kit (STEMCELL technologies, Canada). ..

    Article Title: An immunocompetent human kidney on-a-chip model to study renal inflammation and immune-mediated injury
    Article Snippet: PBMCs were thawed, taken up in Roswell Park Memorial Institute (RPMI) 1640 basal medium (11875093; Thermo Scientific) supplemented with 10% fetal bovine serum (FBS, 16140-071; Gibco), pelleted (300g, 5 min), and washed with phosphate-buffered saline (PBS; 20012068; Sigma) containing 2% FBS. .. Monocytes were isolated from PBMCs by CD14 positive selection using the EasySepTM Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according to manufacturer’s protocol. .. In short, PBMCs were pelleted (300g, 5 min) and resuspended in EasySepTM Buffer (20144; Stemcell Technologies) at a concentration of 1 x 10 cells/mL and incubated with 100 μL selection antibody cocktail per mL of sample for 10 min at RT.

    Article Title: Acetaldehyde exposure underlies functional defects in monocytes induced by excessive alcohol consumption
    Article Snippet: The following antibodies were used for cell surface staining: PE-Cy7 Mouse Anti-Human CD14 (BD Pharmingen), APC-Cy7 Mouse Anti-Human CD16 (BD Pharmingen), FITC Mouse Anti-Human CD3 (BD Pharmingen), PE-Cy7 Mouse Anti-Human CD56 (BD Pharmingen), APC anti-human CD303 (BDCA-2) Antibody (BioLegend), and PerCP/Cyanine5.5 anti-human CD123 Antibody (BioLegend). .. CD14 + monocytes were isolated by immunomagnetic positive selection using the EasySepTM Human CD14 Positive Selection Kit (STEMCELL technologies, Canada). ..

    Centrifugation:

    Article Title: Identification of Key Genes as Early Warning Signals of Acute Myocardial Infarction Based on Weighted Gene Correlation Network Analysis and Dynamic Network Biomarker Algorithm.
    Article Snippet: .. Leukocytes were isolated by centrifugation, and monocytes were isolated by using EasySepTM Human CD14 Positive Selection Kit (#18058, Stemcell, USA) following the manufacturer’s instructions. .. RNA was extracted from leukocytes by using TRIzol® Reagent (#15596018, Invitrogen, USA) and frozen at −80°C.

    Ex Vivo:

    Article Title: Role of CD44 + CCR2 + CD64 - monocyte-derived macrophage in chronic rhinosinusitis with nasal polyps.
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    Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from <t>CD14</t> + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .
    Easyseptm Human Cd14 Positive Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easyseptm+human+cd14+positive+selection+kit/easysep+human+t+cell+isolation+kit/pmc12268675-89-0-8
    Average 90 stars, based on 1 article reviews
    easyseptm human cd14 positive selection kit ii - by Bioz Stars, 2026-10
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    easyseptm human cd14 positive selection kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc easyseptm human cd14 positive selection kit
    Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from <t>CD14</t> + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .
    Easyseptm Human Cd14 Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    STEMCELL Technologies Inc easyseptm human cd14+ positive selection kit ii
    Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from <t>CD14</t> + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .
    Easyseptm Human Cd14+ Positive Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easyseptm+human+cd14+positive+selection+kit/easysep+human+t+cell+isolation+kit/pmc11394489-83-10-25
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    easyseptm human cd14 positive selection kit ii  (StemCells Inc)
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    StemCells Inc easyseptm human cd14 positive selection kit ii
    Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from <t>CD14</t> + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .
    Easyseptm Human Cd14 Positive Selection Kit Ii, supplied by StemCells Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easyseptm+human+cd14+positive+selection+kit/easysep+direct+human+neutrophil+isolation+kit/pm39138521-61-9-19
    Average 90 stars, based on 1 article reviews
    easyseptm human cd14 positive selection kit ii - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from CD14 + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .

    Journal: iScience

    Article Title: HIV-1 infection of macrophages differentially primes NK-cell cytotoxicity and proinflammatory cytokine production

    doi: 10.1016/j.isci.2025.112879

    Figure Lengend Snippet: Phenotypic changes of monocyte-derived macrophages after in vitro HIV-1 infection (A) Differentiation of monocyte-derived macrophages (MDM) from CD14 + monocytes of HIV-1(−) donors using M-CSF for seven days and HIV-1 infection with strains 89.6 and THRO.c for six days or stimulation with LPS and IFNγ for 48 h; stainings were performed at day 11 of MDM differentiation. (B) Representative flow cytometry staining of phenotypic MDM markers and gating for HIV-1-infected MDM (CD4 − p24+). (C) Representative immunofluorescent images of unstimulated and HIV-1-89.6-infected MDM stained for Iba-1 (yellow), HIV-1 p24 (magenta), and DAPI (cell nuclei, cyan). Scale bar: 50 μm. For staining antibody information see Table S2. (D) MDM infection frequencies 6 days after HIV-1 infection with 89.6 ( n = 9) and THRO.c ( n = 11). (E) Representative histograms of surface CD80 expression on MDM and log 2 fold change to unstimulated. (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11). (F) Histograms of intracellular pSTAT1 staining and log 2 fold change to unstimulated. (LPS/IFNγ: n = 3; 89.6: n = 7). (G) Contour plot of pSTAT1 in MDM infected with 89.6 (left). RFI values of HIV-1-infected (CD4 − p24+) and bystander (CD4 + p24-) MDM (middle). Relation between pSTAT1 relative fluorescence intensity (RFI) in HIV-1-infected MDM (CD4 − p24+) and the corresponding infection frequency (right). Spearman correlation coefficient was used to analyze the correlation between infection frequency and RFI of pSTAT1 ( n = 7). (H and I) Representative histograms and log 2 fold change to unstimulated of CD48 (LPS/IFNγ: n = 13; 89.6: n = 9; THRO.c: n = 11) and NKG2D ligands (MIC-A/B, ULBP2/5/6) compared to unstimulated MDM (LPS/IFNγ: n = 10; 89.6: n = 5; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. (E, F, H, I) Differences were analyzed using the Wilcoxon signed-rank test against the value 0 with FDR correction (Original Benjamini and Hochberg). Donor numbers vary due to stringent technical exclusion criteria (see Methods). Stainings were performed with antibody panels A and E (B, D, E, H), panel A (I NKG2DL-BV650 ) or panel E (F, G, I NKG2DL-APC) . See also and .

    Article Snippet: EasySepTM Human CD14 Positive Selection Kit II , StemCell Technologies , Cat#17858.

    Techniques: Derivative Assay, In Vitro, Infection, Flow Cytometry, Staining, Expressing, Fluorescence

    Cell-to-cell contact and soluble priming factors on MDM are modulated by HIV-1 infection as well as NK cells (A) Experimental setup of MDM-NK cell co-culture. After 5 days of HIV-1 infection or 24 h of LPS/IFNγ-stimulation of MDM, autologous NK cells were added to the culture at a ratio of 1:1, according to the seeding density of CD14 + monocytes. After 24 h, NK-cell ligand expression on MDM was assessed. (B–D) MDM infection frequencies (CD4 − p24+ cells) after HIV-1 infection with 89.6 and THRO.c with and without 24 h NK-cell co-culture are depicted. Differences in infection frequencies between MDM with and without NK cell co-culture for each strain were analyzed using the Wilcoxon signed-rank test (89.6: n = 8; THRO.c: n = 9). Representative histogram of HLA-E (C) and HLA-ABC (D) surface expression on unstimulated MDM (left). Median fluorescence intensity (MdFI) of HLA-E and HLA-ABC on unstimulated, HIV-1-infected and bystander MDM is compared between MDM cultured without (NK-) or with NK cells (NK+) (right). Differences in MdFI values between infected and bystander MDM and between NK- and NK + MDM in each strain were analyzed using the Wilcoxon signed-rank test with FDR correction (Original Benjamini and Hochberg) (unstained (us): n = 19; unstim.: n = 21; 89.6 NK+: n = 14; 89.6 NK-: n = 9; THRO.c NK+: n = 11; THRO.c NK-: n = 10). (E–G) Scheme showing the cytokine analysis of supernatants from 24 h MDM-NK cell co-culture supernatants. Soluble IL-15 (F), IL-23 (G), and IL-18 (H, left) concentrations in MDM-NK cell co-culture supernatants from HIV-1 89.6, THRO.c and LPS/IFNγ conditions compared to unstimulated co-cultures. Differences in cytokine concentrations to unstimulated were analyzed using the Wilcoxon signed-rank test with FDR correction (Original Benjamini and Hochberg) (89.6: n = 4; THRO.c: n = 9; LPS/IFNγ: n = 10). Only significant p -values are shown. (H, right) Relation between IL-18 concentrations in 89.6- and THRO.c-infected MDM-NK co-cultures and the corresponding infection frequency. Spearman correlation coefficient was used to analyze the correlation between infection frequency and cytokine concentration (89.6: n = 4; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. Stainings were performed with antibody panels A and E (B, C right, D) and panel H (C left) . n.s., nonsignificant. See also .

    Journal: iScience

    Article Title: HIV-1 infection of macrophages differentially primes NK-cell cytotoxicity and proinflammatory cytokine production

    doi: 10.1016/j.isci.2025.112879

    Figure Lengend Snippet: Cell-to-cell contact and soluble priming factors on MDM are modulated by HIV-1 infection as well as NK cells (A) Experimental setup of MDM-NK cell co-culture. After 5 days of HIV-1 infection or 24 h of LPS/IFNγ-stimulation of MDM, autologous NK cells were added to the culture at a ratio of 1:1, according to the seeding density of CD14 + monocytes. After 24 h, NK-cell ligand expression on MDM was assessed. (B–D) MDM infection frequencies (CD4 − p24+ cells) after HIV-1 infection with 89.6 and THRO.c with and without 24 h NK-cell co-culture are depicted. Differences in infection frequencies between MDM with and without NK cell co-culture for each strain were analyzed using the Wilcoxon signed-rank test (89.6: n = 8; THRO.c: n = 9). Representative histogram of HLA-E (C) and HLA-ABC (D) surface expression on unstimulated MDM (left). Median fluorescence intensity (MdFI) of HLA-E and HLA-ABC on unstimulated, HIV-1-infected and bystander MDM is compared between MDM cultured without (NK-) or with NK cells (NK+) (right). Differences in MdFI values between infected and bystander MDM and between NK- and NK + MDM in each strain were analyzed using the Wilcoxon signed-rank test with FDR correction (Original Benjamini and Hochberg) (unstained (us): n = 19; unstim.: n = 21; 89.6 NK+: n = 14; 89.6 NK-: n = 9; THRO.c NK+: n = 11; THRO.c NK-: n = 10). (E–G) Scheme showing the cytokine analysis of supernatants from 24 h MDM-NK cell co-culture supernatants. Soluble IL-15 (F), IL-23 (G), and IL-18 (H, left) concentrations in MDM-NK cell co-culture supernatants from HIV-1 89.6, THRO.c and LPS/IFNγ conditions compared to unstimulated co-cultures. Differences in cytokine concentrations to unstimulated were analyzed using the Wilcoxon signed-rank test with FDR correction (Original Benjamini and Hochberg) (89.6: n = 4; THRO.c: n = 9; LPS/IFNγ: n = 10). Only significant p -values are shown. (H, right) Relation between IL-18 concentrations in 89.6- and THRO.c-infected MDM-NK co-cultures and the corresponding infection frequency. Spearman correlation coefficient was used to analyze the correlation between infection frequency and cytokine concentration (89.6: n = 4; THRO.c: n = 9). All data are shown as median ± IQR with each dot representing one biological replicate. Stainings were performed with antibody panels A and E (B, C right, D) and panel H (C left) . n.s., nonsignificant. See also .

    Article Snippet: EasySepTM Human CD14 Positive Selection Kit II , StemCell Technologies , Cat#17858.

    Techniques: Infection, Co-Culture Assay, Expressing, Fluorescence, Cell Culture, Concentration Assay